Immunotation of Rv2753c (dihydrodipicolinate synthase)
From DrugPedia: A Wikipedia for Drug discovery
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====ABCpred Results==== | ====ABCpred Results==== | ||
| - | Link to [http://www.imtech.res.in/raghava/bcepred/ ABCpred] | + | Link to [http://www.imtech.res.in/raghava/bcepred/ ABCpred]<br> |
Result Predicited by [http://crdd.osdd.net/drugpedia/images/Abcpred_rv2753c.pdf Predicited by ABCPred]<br> | Result Predicited by [http://crdd.osdd.net/drugpedia/images/Abcpred_rv2753c.pdf Predicited by ABCPred]<br> | ||
Revision as of 07:20, 5 January 2010
| Immunotation of Rv2753c (dihydrodipicolinate synthase)
| |
| Name | |
| Dihydrodipicolinate Synthase | |
| Identifiers | |
| Swiss Prot | |
| Genbank | |
| PDB | |
| Chemical data | |
| Formula | C28H37ClN4O6 [1] |
| Mol. wt. | 30,858 Da |
| Pharmacokinetic data | |
| Bioavailability | ? |
| Solubility | ? |
| Isoelectric-Point | 5.57 |
Contents |
General
dapA catalyzes the formation of dihydrodipicolinate from L-aspartate 4-semialdehyde and pyruvate.
L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2H2O.
This protein is eesential for optimal growth of M.tb and found in the cytoplasm as predicted by the WoLFPSORT and Subloc Server. HMMpfam found one domain PF00701 and has Helix Turn Helix secondary structure.
Protein Sequence
>Rv2753c, TB.seq 3066222:3067121 MW:30827 VTTVGFDVAARLGTLLTAMVTPFSGDGSLDTATAARLANHLVDQGCDGLVVSGTTGESPTTTDGEKIELLRAVLEAVGDR ARVIAGAGTYDTAHSIRLAKACAAEGAHGLLVVTPYYSKPPQRGLQAHFTAVADATELPMLLYDIPGRSAVPIEPDTIRA LASHPNIVGVKDAKADLHSGAQIMADTGLAYYSGDDALNLPWLAMGATGFISVIAHLAAGQLRELLSAFGSGDIATARKI NIAVAPLCNAMSRLGGVTLSKAGLRLQGIDVGDPRLPQVAATPEQIDALAADMRAASVLR
Human Homologue Blast Result
| subject ids | % identity | % positives | alignment length | evalue |
| sp|Q9BXD5 | 28 | 46.33 | 300 | 3.00E-023 |
| sp|Q86XE5 | 26.57 | 46.15 | 286 | 4.00E-022 |
| sp|Q9UG63 | 26.49 | 37.75 | 151 | 0.45 |
| sp|O14556 | 22.09 | 50 | 86 | 2 |
| sp|Q7Z7B0 | 27.69 | 44.62 | 65 | 2.7 |
Antigens
Epitopes
B-Cell Epitopes
BCEpred
Link to bcelpep
Result Predicited by Predicited by ABCPred
ABCpred Results
Link to ABCpred
Result Predicited by Predicited by ABCPred
IEBB Analysis
Result Predicted by IEDB
Result Predicited by Predicited by IEDB
MHC class I restricted T cell epitopes
CTL Epitopes
MHC Binder
Class-I Binder
Result Predicited by Predicited by nHLApred
Result Predicted by
nHLApred
| Epitope | No of Alleles |
| VPIEPDTIR | 15 |
| TPFSGDGSL | 14 |
| TLSKAGLRL | 13 |
| IEPDTIRAL | 13 |
| GTYDTAHSI | 13 |
| DDALNLPWL | 11 |
| GFISVIAHL | 10 |
| GATGFISVI | 10 |
| ATPEQIDAL | 10 |
| YDTAHSIRL | 9 |
| GDIATARKI | 9 |
| AVLEAVGDR | 9 |
| ASHPNIVGV | 9 |
| QRGLQAHFT | 7 |
| GLQAHFTAV | 7 |
| AVGDRARVI | 7 |
| YSGDDALNL | 6 |
| VGFDVAARL | 6 |
| QLRELLSAF | 6 |
| TAVADATEL | 5 |
| TAARLANHL | 5 |
| LLRAVLEAV | 5 |
| GEKIELLRA | 5 |
| DTATAARLA | 5 |
| AAGQLRELL | 5 |
| TDGEKIELL | 4 |
| QAHFTAVAD | 4 |
| MSRLGGVTL | 4 |
| LDTATAARL | 4 |
| HPNIVGVKD | 4 |
| GVKDAKADL | 4 |
| DGEKIELLR | 4 |
| KIELLRAVL | 3 |
| HSIRLAKAC | 3 |
| GSGDIATAR | 3 |
| AHLAAGQLR | 3 |
| PTTTDGEKI | 2 |
| IAHLAAGQL | 2 |
| DLHSGAQIM | 2 |
Class-II Binder
Result predicted by NetMHCII
| Epitope | No of Alleles |
| TGFISVIAHLAAGQL | 6 |
| GFISVIAHLAAGQLR | 6 |
| PDTIRALASHPNIVG | 4 |
| GATGFISVIAHLAAG | 4 |
| ATGFISVIAHLAAGQ | 4 |
| TELPMLLYDIPGRSA | 3 |
| MGATGFISVIAHLAA | 3 |
| LVVTPYYSKPPQRGL | 3 |
| GQLRELLSAFGSGDI | 3 |
| FISVIAHLAAGQLRE | 3 |
| DTIRALASHPNIVGV | 3 |
| QLRELLSAFGSGDIA | 2 |
| PEQIDALAADMRAAS | 2 |
| NLPWLAMGATGFISV | 2 |
| LPWLAMGATGFISVI | 2 |
| LNLPWLAMGATGFIS | 2 |
| LLVVTPYYSKPPQRG | 2 |
| LGGVTLSKAGLRLQG | 2 |
| KAGLRLQGIDVGDPR | 2 |
| IAGAGTYDTAHSIRL | 2 |
| GLLVVTPYYSKPPQR | 2 |
| GGVTLSKAGLRLQGI | 2 |
| EPDTIRALASHPNIV | 2 |
| ELPMLLYDIPGRSAV | 2 |
| ATELPMLLYDIPGRS | 2 |
| ALNLPWLAMGATGFI | 2 |
| AGQLRELLSAFGSGD | 2 |
| AGLRLQGIDVGDPRL | 2 |
