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Revision as of 08:06, 17 December 2009

Immunotation of Rv2753c (dihydrodipicolinate synthase)
Name
Dihydrodipicolinate Synthase
Identifiers
Swiss Prot	P63945
Genbank	888289
PDB	1XXX
Chemical data
Formula	?
Mol. wt.	30,858 Da
Pharmacokinetic data
Bioavailability	?
Solubility	?
Isoelectric-Point	5.57

General

dapA catalyzes the formation of dihydrodipicolinate from L-aspartate 4-semialdehyde and pyruvate.

L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2H₂O.

This protein is eesential for optimal growth of M.tb and found in the cytoplasm as predicted by the WoLFPSORT and Subloc Server. HMMpfam found one domain PF00701 and has Helix Turn Helix secondary structure.

Human Homologue Blast Result

subject ids	% identity	% positives	alignment length	evalue
sp\|Q9BXD5	28	46.33	300	3.00E-023
sp\|Q86XE5	26.57	46.15	286	4.00E-022
sp\|Q9UG63	26.49	37.75	151	0.45
sp\|O14556	22.09	50	86	2
sp\|Q7Z7B0	27.69	44.62	65	2.7

Antigens

Haptens

Epitopes

B-Cell Epitopes

PFSGDGSLDTA

GTTGESPTTTDGE

GTYDTAH

AEG

YSKPPQRGLQ

DATEL

RSAVPIEPDTIRA

KADLHSG

YSGDDALN

IATAR

GDPRLPQVAATPEQIDALAAD

MHC class I restricted T cell epitopes

CTL Epitopes

MHC Binder

Class-I Binder

Epitope	No of Alleles
VPIEPDTIR	15
TPFSGDGSL	14
TLSKAGLRL	13
IEPDTIRAL	13
GTYDTAHSI	13
DDALNLPWL	11
GFISVIAHL	10
GATGFISVI	10
ATPEQIDAL	10
YDTAHSIRL	9
GDIATARKI	9
AVLEAVGDR	9
ASHPNIVGV	9
QRGLQAHFT	7
GLQAHFTAV	7
AVGDRARVI	7
YSGDDALNL	6
VGFDVAARL	6
QLRELLSAF	6
TAVADATEL	5
TAARLANHL	5
LLRAVLEAV	5
GEKIELLRA	5
DTATAARLA	5
AAGQLRELL	5
TDGEKIELL	4
QAHFTAVAD	4
MSRLGGVTL	4
LDTATAARL	4
HPNIVGVKD	4
GVKDAKADL	4
DGEKIELLR	4
KIELLRAVL	3
HSIRLAKAC	3
GSGDIATAR	3
AHLAAGQLR	3
PTTTDGEKI	2
IAHLAAGQL	2
DLHSGAQIM	2

Class-II Binder

Result predicted by NetMHCII

Epitope	No of Alleles
TGFISVIAHLAAGQL	6
GFISVIAHLAAGQLR	6
PDTIRALASHPNIVG	4
GATGFISVIAHLAAG	4
ATGFISVIAHLAAGQ	4
TELPMLLYDIPGRSA	3
MGATGFISVIAHLAA	3
LVVTPYYSKPPQRGL	3
GQLRELLSAFGSGDI	3
FISVIAHLAAGQLRE	3
DTIRALASHPNIVGV	3
QLRELLSAFGSGDIA	2
PEQIDALAADMRAAS	2
NLPWLAMGATGFISV	2
LPWLAMGATGFISVI	2
LNLPWLAMGATGFIS	2
LLVVTPYYSKPPQRG	2
LGGVTLSKAGLRLQG	2
KAGLRLQGIDVGDPR	2
IAGAGTYDTAHSIRL	2
GLLVVTPYYSKPPQR	2
GGVTLSKAGLRLQGI	2
EPDTIRALASHPNIV	2
ELPMLLYDIPGRSAV	2
ATELPMLLYDIPGRS	2
ALNLPWLAMGATGFI	2
AGQLRELLSAFGSGD	2
AGLRLQGIDVGDPRL	2

</td></tr>

Epitope	No of Alleles
IRLAKACAA	51
INIAVAPLC	51
VVSGTTGES	48
LLVVTPYYS	48
IRALASHPN	46
LTAMVTPFS	44
LQAHFTAVA	43
LGTLLTAMV	43
LLTAMVTPF	42
VAPLCNAMS	41
IATARKINI	40
LRELLSAFG	38
LLYDIPGRS	38
LVVSGTTGE	37
VIAHLAAGQ	36
MLLYDIPGR	36
LRLQGIDVG	35
ISVIAHLAA	34
LQGIDVGDP	33
FISVIAHLA	33
WLAMGATGF	32
LLRAVLEAV	30
IELLRAVLE	30
YYSKPPQRG	25
IAVAPLCNA	24
LAADMRAAS	22
IVGVKDAKA	22
LCNAMSRLG	21
LANHLVDQG	21
LAMGATGFI	21
FGSGDIATA	20
YSGDDALNL	19
VGFDVAARL	18
LRAVLEAVG	17
LGGVTLSKA	17
IEPDTIRAL	17
LVVTPYYSK	16
LSKAGLRLQ	16
LHSGAQIMA	16
IMADTGLAY	16
IAHLAAGQL	16
VGVKDAKAD	15
MVTPFSGDG	15
LASHPNIVG	15
FSGDGSLDT	14
LNLPWLAMG	13
VGDRARVIA	12
IAGAGTYDT	11
FDVAARLGT	11
IPGRSAVPI	10
VTPYYSKPP	9
MSRLGGVTL	9
LSAFGSGDI	9
YDTAHSIRL	8
VTLSKAGLR	6
MGATGFISV	5
LYDIPGRSA	5
LVDQGCDGL	5
LAYYSGDDA	5
VAARLGTLL	4
LDTATAARL	4
VTPFSGDGS	3
VPIEPDTIR	3
LLSAFGSGD	3
LAKACAAEG	3
YSKPPQRGL	2
YDIPGRSAV	2
VVTPYYSKP	2
LPQVAATPE	2
LEAVGDRAR	2
LAAGQLREL	2

Alergen Protein

Non Allergen Predicted by AlgPred Server

Antibacterial

Bacterial Toxin

External Links

@@ Line 210: / Line 210: @@
 </td></tr><table>
+<table border='1'>
 </td></tr><tr><td>
-Epitope: No of Alleles
+Epitope</td><td> No of Alleles
 </td></tr><tr><td>
-IRLAKACAA       51
+IRLAKACAA  </td><td>     51
 </td></tr><tr><td>
-INIAVAPLC       51
+INIAVAPLC  </td><td>     51
 </td></tr><tr><td>
-VVSGTTGES       48
+VVSGTTGES  </td><td>     48
 </td></tr><tr><td>
-LLVVTPYYS       48
+LLVVTPYYS </td><td>      48
 </td></tr><tr><td>
-IRALASHPN       46
+IRALASHPN </td><td>      46
 </td></tr><tr><td>
-LTAMVTPFS       44
+LTAMVTPFS  </td><td>     44
 </td></tr><tr><td>
-LQAHFTAVA       43
+LQAHFTAVA  </td><td>     43
 </td></tr><tr><td>
-LGTLLTAMV       43
+LGTLLTAMV  </td><td>     43
 </td></tr><tr><td>
-LLTAMVTPF       42
+LLTAMVTPF  </td><td>     42
 </td></tr><tr><td>
-VAPLCNAMS       41
+VAPLCNAMS  </td><td>     41
 </td></tr><tr><td>
-IATARKINI       40
+IATARKINI </td><td>      40
 </td></tr><tr><td>
-LRELLSAFG       38
+LRELLSAFG </td><td>      38
 </td></tr><tr><td>
-LLYDIPGRS       38
+LLYDIPGRS  </td><td>     38
 </td></tr><tr><td>
-LVVSGTTGE       37
+LVVSGTTGE  </td><td>     37
 </td></tr><tr><td>
-VIAHLAAGQ       36
+VIAHLAAGQ  </td><td>     36
 </td></tr><tr><td>
-MLLYDIPGR       36
+MLLYDIPGR  </td><td>     36
 </td></tr><tr><td>
-LRLQGIDVG       35
+LRLQGIDVG  </td><td>     35
 </td></tr><tr><td>
-ISVIAHLAA       34
+ISVIAHLAA  </td><td>     34
 </td></tr><tr><td>
-LQGIDVGDP       33
+LQGIDVGDP  </td><td>     33
 </td></tr><tr><td>
-FISVIAHLA       33
+FISVIAHLA </td><td>      33
 </td></tr><tr><td>
-WLAMGATGF       32
+WLAMGATGF  </td><td>     32
 </td></tr><tr><td>
-LLRAVLEAV       30
+LLRAVLEAV  </td><td>     30
 </td></tr><tr><td>
-IELLRAVLE       30
+IELLRAVLE  </td><td>     30
 </td></tr><tr><td>
-YYSKPPQRG       25
+YYSKPPQRG  </td><td>     25
 </td></tr><tr><td>
-IAVAPLCNA       24
+IAVAPLCNA </td><td>      24
 </td></tr><tr><td>
-LAADMRAAS       22
+LAADMRAAS </td><td>      22
 </td></tr><tr><td>
-IVGVKDAKA       22
+IVGVKDAKA </td><td>      22
 </td></tr><tr><td>
-LCNAMSRLG       21
+LCNAMSRLG </td><td>      21
 </td></tr><tr><td>
-LANHLVDQG       21
+LANHLVDQG  </td><td>     21
 </td></tr><tr><td>
-LAMGATGFI       21
+LAMGATGFI  </td><td>     21
 </td></tr><tr><td>
-FGSGDIATA       20
+FGSGDIATA  </td><td>     20
 </td></tr><tr><td>
-YSGDDALNL       19
+YSGDDALNL </td><td>      19
 </td></tr><tr><td>
-VGFDVAARL       18
+VGFDVAARL </td><td>      18
 </td></tr><tr><td>
-LRAVLEAVG       17
+LRAVLEAVG </td><td>      17
 </td></tr><tr><td>
-LGGVTLSKA       17
+LGGVTLSKA </td><td>      17
 </td></tr><tr><td>
-IEPDTIRAL       17
+IEPDTIRAL </td><td>      17
 </td></tr><tr><td>
-LVVTPYYSK       16
+LVVTPYYSK </td><td>      16
 </td></tr><tr><td>
-LSKAGLRLQ       16
+LSKAGLRLQ  </td><td>     16
 </td></tr><tr><td>
-LHSGAQIMA       16
+LHSGAQIMA </td><td>      16
 </td></tr><tr><td>
-IMADTGLAY       16
+IMADTGLAY </td><td>      16
 </td></tr><tr><td>
-IAHLAAGQL       16
+IAHLAAGQL  </td><td>     16
 </td></tr><tr><td>
-VGVKDAKAD       15
+VGVKDAKAD </td><td>      15
 </td></tr><tr><td>
-MVTPFSGDG       15
+MVTPFSGDG  </td><td>     15
 </td></tr><tr><td>
-LASHPNIVG       15
+LASHPNIVG </td><td>      15
 </td></tr><tr><td>
-FSGDGSLDT       14
+FSGDGSLDT  </td><td>     14
 </td></tr><tr><td>
-LNLPWLAMG       13
+LNLPWLAMG </td><td>      13
 </td></tr><tr><td>
-VGDRARVIA       12
+VGDRARVIA </td><td>      12
 </td></tr><tr><td>
-IAGAGTYDT       11
+IAGAGTYDT </td><td>      11
 </td></tr><tr><td>
-FDVAARLGT       11
+FDVAARLGT </td><td>      11
 </td></tr><tr><td>
-IPGRSAVPI       10
+IPGRSAVPI </td><td>      10
 </td></tr><tr><td>
-VTPYYSKPP       9
+VTPYYSKPP  </td><td>     9
 </td></tr><tr><td>
-MSRLGGVTL       9
+MSRLGGVTL </td><td>      9
 </td></tr><tr><td>
-LSAFGSGDI       9
+LSAFGSGDI  </td><td>     9
 </td></tr><tr><td>
-YDTAHSIRL       8
+YDTAHSIRL </td><td>      8
 </td></tr><tr><td>
-VTLSKAGLR       6
+VTLSKAGLR </td><td>      6
 </td></tr><tr><td>
-MGATGFISV       5
+MGATGFISV  </td><td>     5
 </td></tr><tr><td>
-LYDIPGRSA       5
+LYDIPGRSA  </td><td>     5
 </td></tr><tr><td>
-LVDQGCDGL       5
+LVDQGCDGL </td><td>      5
 </td></tr><tr><td>
-LAYYSGDDA       5
+LAYYSGDDA </td><td>      5
 </td></tr><tr><td>
-VAARLGTLL       4
+VAARLGTLL </td><td>      4
 </td></tr><tr><td>
-LDTATAARL       4
+LDTATAARL </td><td>      4
 </td></tr><tr><td>
-VTPFSGDGS       3
+VTPFSGDGS  </td><td>     3
 </td></tr><tr><td>
-VPIEPDTIR       3
+VPIEPDTIR  </td><td>     3
 </td></tr><tr><td>
-LLSAFGSGD       3
+LLSAFGSGD  </td><td>     3
 </td></tr><tr><td>
-LAKACAAEG       3
+LAKACAAEG  </td><td>     3
 </td></tr><tr><td>
-YSKPPQRGL       2
+YSKPPQRGL </td><td>      2
 </td></tr><tr><td>
-YDIPGRSAV       2
+YDIPGRSAV </td><td>      2
 </td></tr><tr><td>
-VVTPYYSKP       2
+VVTPYYSKP  </td><td>     2
 </td></tr><tr><td>
-LPQVAATPE       2
+LPQVAATPE </td><td>      2
 </td></tr><tr><td>
-LEAVGDRAR       2
+LEAVGDRAR  </td><td>     2
 </td></tr><tr><td>
-LAAGQLREL       2
+LAAGQLREL  </td><td>     2
 </td></tr><table>

Immunotation of Rv2753c (dihydrodipicolinate synthase)