Zincfinger
From DrugPedia: A Wikipedia for Drug discovery
(New page: WHAT IS ZINC FINGER? Configuration of a DNA-binding protein that resembles a finger with a base, usually cysteines and histidines, binding a zinc ion. Discovered in a transcription factor...) |
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| - | WHAT IS ZINC FINGER? | + | '''WHAT IS ZINC FINGER?''' |
Configuration of a DNA-binding protein that resembles a finger with a base, usually cysteines and histidines, binding a zinc ion. Discovered in a transcription factor in Xenopus but present in a large number of different proteins. | Configuration of a DNA-binding protein that resembles a finger with a base, usually cysteines and histidines, binding a zinc ion. Discovered in a transcription factor in Xenopus but present in a large number of different proteins. | ||
| - | Function: | + | '''Function:''' |
The DNA-binding motif is found as part of transcription regulatory proteins. | The DNA-binding motif is found as part of transcription regulatory proteins. | ||
| - | Structure: | + | '''Structure:''' |
One of the most abundant DNA-binding motifs. Proteins may contain more than one finger in a single chain; each motif consists of 2 antiparallel beta-strands followed by by an alpha-helix. A single zinc ion is tetrahedrally coordinated by conserved histidine and cysteine residues, stabilising the motif. | One of the most abundant DNA-binding motifs. Proteins may contain more than one finger in a single chain; each motif consists of 2 antiparallel beta-strands followed by by an alpha-helix. A single zinc ion is tetrahedrally coordinated by conserved histidine and cysteine residues, stabilising the motif. | ||
| - | Binding: | + | '''Binding:''' |
Fingers bind to 3 base-pair subsites and specific contacts are mediated by amino acids in positions -1, 2, 3 and 6 relative to the start of the alpha-helix. Contacts mainly involve one strand of the DNA.Where proteins contain multiple fingers, each finger binds to adjacent subsites within a larger DNA recognition site thus allowing a relatively simple motif to specifically bind to a wide range of DNA sequences. | Fingers bind to 3 base-pair subsites and specific contacts are mediated by amino acids in positions -1, 2, 3 and 6 relative to the start of the alpha-helix. Contacts mainly involve one strand of the DNA.Where proteins contain multiple fingers, each finger binds to adjacent subsites within a larger DNA recognition site thus allowing a relatively simple motif to specifically bind to a wide range of DNA sequences. | ||
Current revision
WHAT IS ZINC FINGER?
Configuration of a DNA-binding protein that resembles a finger with a base, usually cysteines and histidines, binding a zinc ion. Discovered in a transcription factor in Xenopus but present in a large number of different proteins.
Function: The DNA-binding motif is found as part of transcription regulatory proteins.
Structure: One of the most abundant DNA-binding motifs. Proteins may contain more than one finger in a single chain; each motif consists of 2 antiparallel beta-strands followed by by an alpha-helix. A single zinc ion is tetrahedrally coordinated by conserved histidine and cysteine residues, stabilising the motif.
Binding: Fingers bind to 3 base-pair subsites and specific contacts are mediated by amino acids in positions -1, 2, 3 and 6 relative to the start of the alpha-helix. Contacts mainly involve one strand of the DNA.Where proteins contain multiple fingers, each finger binds to adjacent subsites within a larger DNA recognition site thus allowing a relatively simple motif to specifically bind to a wide range of DNA sequences.
[edit] EXTERNAL LINKS
[1] (zinc finger consortium)
[2] (BI tools related to searching zinc fingers)
